DNA sequencing service 
 Co-star Biotechnology Co., Ltd. has advanced sequencing instruments, science and technology and rich experience. From quality to cycle with, comprehensive, multi-angle meet customer requirements for different levels of sequencing capacity. We can receive strains, plasmids, PCR products were sequenced and other samples. 

 Service advantages: 

 Using ABI 3730xl sequencer and corresponding kits. 
 For the high-GC, hairpin, PloyA / T and so has extensive experience in handling complex template and has maintained a high success rate of the experiment. 
 Extraction and purification of all the use of imported Axygen supplies, PCR products were purified by tapping without exception, to ensure a high purity of DNA, a normal reaction to read 800 bases. 2-3 working days receipt of the samples sent by E-mail report. 
 Sample requirements and primers described 

 Category specific requirements 
 Bacilli 1 provides> 200μl overnight culture around (12h) of the bacilli, stored in sealed Eppendorf tubes, to prevent cross-contamination or leakage (preferably 5ml broth, so we quickly process your sample). 
 (2) a large number of samples sequenced, it is recommended in disposable plastic petri dishes on solid medium puncture training. 
 3 shows the type of carrier resistance, we provide ampicillin, carbenicillin, kanamycin, chloramphenicol, tetracycline and other antibiotics; other types of antibiotics, bring your own. 
 PCR products (already purified) 1. PCR product dissolved in ddH2O (Do not dissolved in TE solution). 
 (2) concentration greater than 50ng/μl, larger than 10μl (only one response, each additional response, provide more 5ul). 
 3. Electrophoresis band specificity. 
 PCR products (unpurified) 1 fragments greater than 100bp. 
 2 Please provide 30-50μl PCR amplification products (20ng/ul, total> 600ng), take 3μl samples, should be able to clearly distinguish the target band 
 Plasmid 1 plasmid dissolved in ddH2O (Do not dissolved in TE solution), electrophoresis concentration greater than 50ng/μl; larger than 10μl (only one response, each additional response, provide more 5ul). 
 (2) must indicate the length of vector and insert fragment, and sequencing requirements. 
 Primers 1 concentration of not less than 3.2pmol/μl, solution volume larger than 10μl; (number of reactions required to provide more and more, under normal circumstances, according to 2ul / a response provided). 
 (2) random primers (RAPD primers) and degenerate primers, mixed primers and primer length of less than 17bp can not be used for sequencing. 
 3 primer length is generally 18-23bp more suitable for sequencing, high specificity. 
 4 provide as much primer sequence, PCR annealing temperature for reference. 

 Sequencing Service Price List 

 Item pricing unit price (yuan) 
 3730 sequencing reaction 35 

 PCR products were purified sample 15