Real-time quantitative PCR (RT-PCR) 
 Real-time quantitative PCR, that is, in the conventional PCR reaction added fluorescent probes or fluorescent dyes, the use of real-time fluorescence PCR monitoring of the entire process, the final adoption of the standard curve of known template for quantitative analysis. Real-time PCR to avoid the traditional quantitative PCR to monitor the end product deviations, to improve the experimental reproducibility. The technology has been widely used to monitor changes in mRNA expression of cells, to compare differences in mRNA expression of the organization to verify gene chip, siRNA interference experiments. 

 Service requirements: 
 Please provide as much fresh and materials. Experiments in the sample (tissue or cell samples recommendations); the amount of tissue sample of not less than 200mg, or about 107 cells, total RNA or mRNA is greater than 5ug than 200ng. 
 Please Email offers full-length gene sequence or a known gene name. 
 Please provide background information as possible, such as DNA / RNA source, abundance and so on. 

 Service program: 
 According to the gene sequences to design specific primers and fluorescent probes. 
 Extraction of DNA / RNA 
 Quantitative PCR, analysis of experimental results 
 And experimental results provide the experimental data reporting 

 Fees: 
 RNA extraction costs :1-20 samples, 140 yuan / month 
 20-30 samples, 100 yuan / month 
 More than 30 samples, 80 yuan / month 
 PCR reaction cost: detection of a target, each sample 140 yuan 
 Detection of two targets, each sample 220 yuan 
 Detection of three indicators, each sample 270 yuan 
 Four or more indicators, each sample 70 / indicators 
 Primers and probe synthesis separately. 
 Completion Time: 2-3 weeks after receipt of the samples