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| No.8549296

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Information Name: | Supply [IFN g] recombinant human interferon-γ |
Published: | 2012-02-01 |
Validity: | 30 |
Specifications: | |
Quantity: | 0.00 |
Price Description: | |
Detailed Product Description: | [IFN g] recombinant human interferon-γ Product Price ¥ 800/20μg ¥ 2080/100μg ¥ 16000/1mg Background Interferon (interferon, IFN) is related to stimulation of cells arising from cell signaling proteins, is a cytokine superfamily glycoproteins homologous cytokine family, known as the IFN family. Has identified the three main family members: IFN-α, IFN-β and IFN-γ. Including interferon-γ (Interferon-gamma, IFN-γ) is a kind of anti-virus, anti-tumor and immunomodulatory effects of cytokines, mainly by activated T cells and NK cells in the immune system play an important role . IFN-γ is a class of secreted glycoproteins, the 143 amino acids, relative molecular weight is 16924. And recombinant human interferon-γ between the relative molecular weight of 14000-16000, which is due to re-IFN-γ in the C-terminal amino acids are missing phenomenon. Product Description Recombinant human interferon-γ recombinant protein was expressed in E. coli, is a single chain, non-glycosylated polypeptide chain containing 144 amino acids, the molecular weight of 16879Da. The recombinant human interferon-γ and ultimately by standard chromatographic techniques and purified. Source host Recombinant expression in E. coli Sequence MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ. Solution proposed Provide human interferon-γ (IFN g) of white filter-sterilized products lyophilized powder; the lyophilized powder containing 1mg/ml from the human interferon-γ (IFN g) of 10mM sodium phosphate buffer (pH 7.4 ± 0.1) in the further dialysis and freeze-dried produce. We recommend additional sterile 18MΩ-cm water for the preparation of working stock solution to 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. Preservation proposal Human interferon-γ (IFN g) lyophilized powder can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed packing (plus carrier protein such as 0.1% HSA or BSA) and placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing. |
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Copyright © GuangDong ICP No. 10089450, Shanghai Hexing Biotechnology Co., Ltd. All rights reserved.
Technical support: ShenZhen AllWays Technology Development Co., Ltd.
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You are the 32851 visitor
Copyright © GuangDong ICP No. 10089450, Shanghai Hexing Biotechnology Co., Ltd. All rights reserved.
Technical support: ShenZhen AllWays Technology Development Co., Ltd.
AllSources Network's Disclaimer: The legitimacy of the enterprise information does not undertake any guarantee responsibility