[BDNF] recombinant human brain-derived neurotrophic factor 
 Product Price 
 ￥ 800/2μg ￥ 2080/10μg ￥ 74880/1mg 

 Background 
 Brain-derived neurotrophic factor (BDNF) was originally purified from porcine brain basic protein molecules, is a neurotrophic factor family members, for a small basic proteins. Different with nerve growth factor, brain-derived neurotrophic factor, mainly in the central nervous system, neural crest and ectoderm substrate to maintain sensory neurons, these neurons on nerve growth factor-free reaction. Brain-derived neurotrophic factor in neuronal survival, differentiation and regulation of synaptic plasticity plays an important role in the brain involved in learning and memory. Biological effects of BDNF mainly mediated through its receptor TrkB. 

 Product Description 
 Human brain-derived neurotrophic factor recombinant proteins in E. coli recombinant expression, and by two non-glycosylated polypeptide chain homodimer consisting of, containing 2 x 119 amino acids, with a total molecular weight of 26,984 Da . The human brain-derived neurotrophic factor recombinant proteins and ultimately by the standard chromatographic techniques and purified. 

 Source host 
 Recombinant expression in E. coli 

 Sequence 
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ser-Asp-Pro. 



 Solution proposed 
 Provide the human brain-derived neurotrophic factor (BDNF) products of white filter-sterilized lyophilized powder; containing the lyophilized powder from the human brain-derived neurotrophic factor (BDNF) in 20mM sodium citrate buffer (pH 5 ) by dialysis and freeze-dried in the generated. We recommend sterile 18MΩ-cm water and dissolved to a concentration of not less than 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. 

 Preservation proposal 
 Human brain-derived neurotrophic factor (BDNF) lyophilized powder can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed packing (plus carrier protein such as 0.1% HSA or BSA) and placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing.