[IL 1 beta] recombinant human interleukin-1β 
 Product Price 
 ￥ 800/2μg ￥ 2080/10μg ￥ 56000/1mg 

 Background 
 Interleukin -1 (interleukin-1, IL-1) infection and inflammation in the state, by a variety of cells, there are many biological functions of cytokines, and its wide range of biological effects, including participation in mediated guide inflammation, immune regulation, regulating body metabolism. IL-1 molecular weight of 17.5KD, there are two types: IL-1α and IL-1β, IL-1β mainly based. IL-1 receptor (interleukin-1 receptor, IL-1R), including type Ⅰ and Ⅱ, there is also an endogenous IL-1 receptor antagonist (interleukin-1 receptor antagonist, IL-1ra or IL- 1γ), IL-1ra with IL-1 receptor, but does not cause a relevant role. Inflammation of the case, granulocyte-macrophage colony-stimulating factor (GM-CSF) levels, it can also promote the role of IL-1 increases, increasing the synthesis of IL-1β; other synovial cells can also be autocrine IL- 1β. 

 Product Description 
 Human recombinant interleukin-1β recombinant protein was expressed in E. coli, is a non-glycosylated polypeptide chain containing 153 amino acids, the molecular weight of 17000Da. The human interleukin-1β recombinant proteins and ultimately by the standard chromatographic techniques and purified. 

 Source host 
 Recombinant expression in E. coli 

 Sequence 
 The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser. 



 Solution proposed 
 Provide human interleukin-1β (IL 1 beta) products are filter-sterilized white lyophilized powder; from the lyophilized powder containing 1mg/ml human interleukin-1β (IL 1 beta) in 50mM phosphate buffer (pH 7.1) and 150mM NaCl solution filter sterilized and freeze-dried produce. We recommend additional sterile 18MΩ-cm water for the preparation of working stock solution to 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. 

 Preservation proposal 
 Human interleukin-1β (IL 1 beta) lyophilized powder can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed packing (plus carrier protein such as 0.1% HSA or BSA) and placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing.