[PDGF BB Yeast] recombinant human platelet-derived growth factor-BB, yeast expression 
 Product Price 
 ￥ 800/2μg ￥ 2080/10μg ￥ 56160/1mg 

 Background 
 Platelet-derived growth factor (Platelet Derived Growth Factor, PDGF) is composed of two related polypeptide chains connected by disulfide bonds formed dimers. PDGF stored in platelet α-granule protein in an alkaline, some cells such as fibroblasts, smooth muscle cells, glial cell mitogen. PDGF can be divided into PDGF-I and PDGF-II, both proteins contain the same, only different glycosides, which is composed of two different polypeptide chains by a combination of disulfide dimer, which compared with A-chain large (PDGF-I in the A chain of 17 000, PDGF-II of the A chain of 15 000), B chain of small (14 000). By the platelet-purified PDGF for the AB, BB dimer, PDGF in many tumor cells of AA dimer. Now that PDGF in wound healing, cholesterol and phospholipid biosynthesis, atherosclerosis and other physiological and pathological processes play an important role. 

 Product Description 
 Human platelet-derived growth factor-BB, recombinant protein expression in the yeast Saccharomyces cerevisiae recombinant, glycosylated by two polypeptide chains of the homodimer, contains 109 amino acids, the molecular weight of 32,021 Da . The human platelet-derived growth factor-BB, the final yeast expression of recombinant proteins purified by standard chromatographic techniques out. 

 Source host 
 Saccharomyces cerevisiae recombinant 

 Sequence 
 The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu. 



 Solution proposed 
 Provide human platelet-derived growth factor-BB, yeast expression (PDGF BB Yeast) products through 0.2μm pore size filter-sterilized, white lyophilized powder; from the lyophilized powder containing human platelet-derived growth factor-BB, yeast expression ( PDGF BB Yeast) in 20mM sodium acetate (without any carrier protein) solution, filter-sterilized and freeze-dried produce. We recommend additional sterile 18MΩ-cm water for the preparation of working stock solution to not less than 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. 

 Preservation proposal 
 Human platelet-derived growth factor-BB, yeast expression (PDGF BB Yeast) can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed aliquot placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing.