[B NGF CHO] recombinant human β-nerve growth factor, CHO expression 
 Product Price 
 ￥ 800/5μg ￥ 2080/20μg ￥ 43200/1mg 

 Background 
 Nerve growth factor (NGF) is a neurotrophic factor in the first to be found, the present study the most thorough, with neurons to promote neurite outgrowth nutrition and biological functions of the dual regulation of neural cell growth factor, its central and peripheral neuronal development, differentiation , growth, regeneration and functional characterization are an important regulatory role. Nerve growth factor containing α, β, γ three subunits, the active area is the β subunit. β-nerve growth factor consists of two 118 amino acid single-chain through a combination of non-covalent dimer, and the structure of the human nerve growth factor with a high degree of homology, biological effects and no significant species-specific. NGF receptor binding, by receptor-mediated endocytosis have internalized, formed by the membrane surrounding the shaft, with NGF, and to maintain its biological activity vesicles by retrograde axonal transport along microtubules to the cell body, the tyrosine acid protein kinases, inositol lipid calcium, and other endogenous cyclic adenosine monophosphate second messenger transduction system, starting a cascade of reactions, some of the structure or function of target cell proteins regulate gene expression and exert their biological effects . 

 Product Description 
 Human β-nerve growth factor, CHO expression of recombinant proteins in CHO Chinese hamster ovary cells expressing the recombinant, glycosylated by two polypeptide chains of the homodimer, each polypeptide chain containing 120 amino acids, molecular weight of 16,950 Da. The human β-nerve growth factor, CHO expression of recombinant proteins and ultimately by standard chromatographic techniques and purified. 

 Source host 
 CHO Chinese hamster ovary cells expressing recombinant 

 Sequence 
 The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-His-Pro. 



 Solution proposed 
 Provide human β-nerve growth factor, CHO expression (b NGF CHO) products through 0.2μm pore size filter-sterilized, white lyophilized powder; from the lyophilized powder containing human β-nerve growth factor, CHO expression (b NGF CHO) in 30mM sodium acetate (pH 5.5), 1% HSA solution, freeze-dried produce. We recommend additional sterile 18MΩ-cm water for the preparation of working stock solution to not less than 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. 

 Preservation proposal 
 Human β-nerve growth factor, CHO expression (b NGF CHO) lyophilized powder can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed aliquot placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing.