[HGF CHO] recombinant human hepatocyte growth factor, CHO expression 
 Product Price 
 ￥ 800/2μg ￥ 2080/10μg ￥ 70400/1mg 

 Background 
 HGF (hepatocyte growth factor, HGF) was originally used as a liver cell mitosis was originally from partial hepatectomy rat serum isolated. HGF is a mesenchymal derived pleiotropic growth factor, can promote a variety of cell division and growth. By paracrine, endocrine, autocrine form of protection organs, play a role in tissue aging extended. HGF first to a non-active single-chain protein (HGF SF) in the form secreted by the hepatocyte growth factor activator cut into mature heterodimeric HGF, HGFA the body can activate HGF SF precursor, so that from the single-chain precursor into double-stranded form of activity, to regulate the activity of extracellular HGF SF key steps. HGF in the same time promote regeneration after injury in normal tissue repair, cell movement and tumor formation, invasion and metastasis, differentiation, and tumor blood vessels play an important role in the trip. 

 Product Description 
 Human hepatocyte growth factor, CHO expression of recombinant proteins in CHO Chinese hamster ovary cells, recombinant expression, and by two non-glycosylated polypeptide chain, that is 463 amino acids of the α chain of 234 amino acids and β-chains of the differences source dimer, with a total molecular weight of 75kDa. The human hepatocyte growth factor, CHO expression of recombinant proteins and ultimately by standard chromatographic techniques and purified. 

 Source host 
 CHO Chinese hamster ovary cells expressing recombinant 

 Sequence 
 Agrees with the sequence of native human HGF 

 Solution proposed 
 Provide human hepatocyte growth factor, CHO expression (HGF CHO) product is filtered sterile white lyophilized powder; the lyophilized powder containing 1mg/ml from the human hepatocyte growth factor, CHO expression (HGF CHO) of 0.02% Tween 80, phosphate buffer (pH 7.4) in the freeze-dried produce. We recommend additional sterile 18MΩ-cm water for the preparation of working stock solution to 100μg/ml, follow-up experiment based on the experimental requirements can be further diluted. 

 Preservation proposal 
 Human hepatocyte growth factor, CHO expression (HGF CHO) lyophilized powder can be stored at room temperature for 3 weeks, or placed in -18 ℃ cryopreservation for long-term preservation. Re-dissolved in 4 ℃ refrigerator for at least 2-7 days; If you want to extend the storage time, the proposed packing (plus carrier protein such as 0.1% HSA or BSA) and placed in -18 ℃ cryopreservation. Avoid repeated freezing and thawing.