(Earthox) Anti-Myc Tag Monoclonal Antibody [Myc.A7] Myc tag antibody, anti-Myc monoclonal antibody, Myc mouse origin monoclonal antibody English name Anti-Myc Tag Monoclonal Antibody [Myc.A7] the Chinese Name Myc tag antibody, anti- Myc monoclonal antibody, Myc mouse monoclonal antibody background information in 1985, developed a mouse anti-c-myc tag antibody and immunochemical reagents for cell biology and protein engineering. The Myc tag is equivalent to the human c-Myc 410-419 peptide (SEQ ID NO: EQKLISEEDL). Myc-tag can be placed on the C-terminal or N-terminal myc recombinant protein at low pH elution conditions tend to reduce the vigor of the protein, and Myc-tag system is widely applied to the detection, but rarely used for purification. Myc-tag has been successfully applied to the WB hybridization, immunoprecipitation IP and flow cytometry. Can therefore be used to detect the expression of the recombinant protein in bacteria, yeast, insect cells and mammalian cells. Application Type WB (1:1000 - 1:10000), ICC / IF (1:200 - 1:800), the IP (1:200) IHC (1:200 - 1:800) immunogen KLH coupled Myc tag polypeptides (i.e., peptides of c-Myc 410-419: EQKLISEEDL) the source host mice reactive the Myc-tag antibody can recognize Myc tag within the cell over-expression of recombinant proteins, including preservation proposal Myc is located in the amino and carboxyl end of the recombinant protein after delivery can be stored at -20 ℃. In order to avoid loss, before you open the lid of the tube to melt antibody and centrifuged. We recommend using packing to avoid repeated freezing and thawing, aliquot and kept for 3 months at 4 ℃